Mouse BMP-9 ELISA Kit PicoKine®

Mouse BMP-9 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Gdf2 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 39 35 34 36 2.27 6.3% Sample 2 220 263 251 256 247 16.43 6.6% Sample 3 419 420 444 379 415 23.32 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 39 35 34 36 2.27 6.3% Sample 1 39 39 35 34 36 2.27 6.3% Sample 2 220 263 251 256 247 16.43 6.6% Sample 2 220 263 251 256 247 16.43 6.6% Sample 3 419 420 444 379 415 23.32 5.6% Sample 3 419 420 444 379 415 23.32 5.6%

Synonyms

Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9; Gdf2; Bmp9

Gene Name

Gdf2

Gene ID

12165

UniProt

Q9WV56

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S319-R428

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 220 419 41 226 402 Mean (pg/ml) 39 220 419 41 226 402 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 Standard deviation 1.71 11.22 31.42 2.05 14.46 30.95 CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7% CV (%) 4.4% 7.7%% 7.5% 5% 6.4% 7.7%

Sensitivity

<10 pg/mL

Function

Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse BMP-9

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth differentiation factor 2

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392 O.D. 0.046 0.133 0.215 0.367 0.657 1.006 1.669 2.392

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.