Rat BMP-6 ELISA Kit PicoKine®

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 286 295 282 248 277 17.8 6.4% Sample 2 1189 1131 1215 1119 1163 39.8 3.4% Sample 3 5032 5144 5474 4947 5149 200.08 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 286 295 282 248 277 17.8 6.4% Sample 1 286 295 282 248 277 17.8 6.4% Sample 2 1189 1131 1215 1119 1163 39.8 3.4% Sample 2 1189 1131 1215 1119 1163 39.8 3.4% Sample 3 5032 5144 5474 4947 5149 200.08 3.8% Sample 3 5032 5144 5474 4947 5149 200.08 3.8%

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 286 1189 5032 301 1196 5027 Standard deviation 18.01 68.96 342.17 20.46 69.36 341.83 CV (%) 5.8%% 5.8% 6.8% 6.8% 5.8% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 286 1189 5032 301 1196 5027 Standard deviation 18.01 68.96 342.17 20.46 69.36 341.83 CV (%) 5.8%% 5.8% 6.8% 6.8% 5.8% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 286 1189 5032 301 1196 5027 Mean (pg/ml) 286 1189 5032 301 1196 5027 Standard deviation 18.01 68.96 342.17 20.46 69.36 341.83 Standard deviation 18.01 68.96 342.17 20.46 69.36 341.83 CV (%) 5.8%% 5.8% 6.8% 6.8% 5.8% 6.8% CV (%) 5.8%% 5.8% 6.8% 6.8% 5.8% 6.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.159 0.254 0.404 0.678 1.093 1.769 2.442 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.159 0.254 0.404 0.678 1.093 1.769 2.442 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.159 0.254 0.404 0.678 1.093 1.769 2.442 O.D. 0.008 0.159 0.254 0.404 0.678 1.093 1.769 2.442

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.