Mouse BMP-6 ELISA Kit PicoKine®

Bone morphogenetic protein 6 is a protein that in humans is encoded by the BMP6 gene. It is mapped to 6p24-p23. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 214 208 247 226 223 14.9 6.6% Sample 2 1829 1835 1824 1804 1823 11.64 0.6% Sample 3 5790 6282 6158 5929 6039 191.9 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 214 208 247 226 223 14.9 6.6% Sample 1 214 208 247 226 223 14.9 6.6% Sample 2 1829 1835 1824 1804 1823 11.64 0.6% Sample 2 1829 1835 1824 1804 1823 11.64 0.6% Sample 3 5790 6282 6158 5929 6039 191.9 3.1% Sample 3 5790 6282 6158 5929 6039 191.9 3.1%

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 214 1829 5790 226 1673 5512 Standard deviation 12.41 74.98 399.51 13.33 86.99 391.35 CV (%) 5.8% 4.1% 6.9% 5.9% 5.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 214 1829 5790 226 1673 5512 Standard deviation 12.41 74.98 399.51 13.33 86.99 391.35 CV (%) 5.8% 4.1% 6.9% 5.9% 5.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 214 1829 5790 226 1673 5512 Mean (pg/ml) 214 1829 5790 226 1673 5512 Standard deviation 12.41 74.98 399.51 13.33 86.99 391.35 Standard deviation 12.41 74.98 399.51 13.33 86.99 391.35 CV (%) 5.8% 4.1% 6.9% 5.9% 5.2% 7.1% CV (%) 5.8% 4.1% 6.9% 5.9% 5.2% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.106 0.159 0.249 0.438 0.781 1.515 2.342 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.106 0.159 0.249 0.438 0.781 1.515 2.342 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.106 0.159 0.249 0.438 0.781 1.515 2.342 O.D. 0.052 0.106 0.159 0.249 0.438 0.781 1.515 2.342

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.