Human MMP-10/Stromelysin-2 ELISA Kit PicoKine®

Human MMP10 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MMP10 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Stromelysin-2 also known as matrix metalloproteinase-10 (MMP-10) or transin-2 is an enzyme that in humans is encoded by the MMP10 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 142 133 133 139 7.08 5% Sample 2 657 650 733 643 670 36.27 5.4% Sample 3 2257 2665 2447 2386 2438 147.54 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 142 133 133 139 7.08 5% Sample 1 150 142 133 133 139 7.08 5% Sample 2 657 650 733 643 670 36.27 5.4% Sample 2 657 650 733 643 670 36.27 5.4% Sample 3 2257 2665 2447 2386 2438 147.54 6% Sample 3 2257 2665 2447 2386 2438 147.54 6%

Synonyms

Stromelysin-2; SL-2; 3.4.24.22; Matrix metalloproteinase-10; MMP-10; Transin-2; MMP10; STMY2

Gene Name

MMP10

Gene ID

4319

UniProt

P09238

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C476

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 657 2257 156 631 2147 Standard deviation 9.75 45.33 162.5 10.76 51.11 173.9 CV (%) 6.5% 6.9% 7.2% 6.9% 8.1% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 657 2257 156 631 2147 Standard deviation 9.75 45.33 162.5 10.76 51.11 173.9 CV (%) 6.5% 6.9% 7.2% 6.9% 8.1% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 657 2257 156 631 2147 Mean (pg/ml) 150 657 2257 156 631 2147 Standard deviation 9.75 45.33 162.5 10.76 51.11 173.9 Standard deviation 9.75 45.33 162.5 10.76 51.11 173.9 CV (%) 6.5% 6.9% 7.2% 6.9% 8.1% 8.1% CV (%) 6.5% 6.9% 7.2% 6.9% 8.1% 8.1%

Sensitivity

<10 pg/mL

Function

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MMP-10

Anticoagulant

Heparin

Gene Name Synonym

Matrix metallopeptidase 10

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.112 0.192 0.389 0.777 1.377 2.028 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.112 0.192 0.389 0.777 1.377 2.028 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.112 0.192 0.389 0.777 1.377 2.028 O.D. 0.025 0.065 0.112 0.192 0.389 0.777 1.377 2.028

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.