Mouse FLT1/VEGFR1 ELISA Kit PicoKine®

Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 211 234 239 223 13.12 5.8% Sample 2 954 976 1025 953 977 29.19 2.9% Sample 3 6138 6399 5724 6389 6162 273.91 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 211 234 239 223 13.12 5.8% Sample 1 210 211 234 239 223 13.12 5.8% Sample 2 954 976 1025 953 977 29.19 2.9% Sample 2 954 976 1025 953 977 29.19 2.9% Sample 3 6138 6399 5724 6389 6162 273.91 4.4% Sample 3 6138 6399 5724 6389 6162 273.91 4.4%

Vascular endothelial growth factor receptor 1; VEGFR-1;2.7.10.1; Embryonic receptor kinase 2; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase receptor FLT; Flt1; Emrk2, Flt, Vegfr1

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Protein Phosphorylation, Response To Hypoxia, Signal Transduction, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1914 6138 191 959 6693 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 CV (%) 4.2% 7% 5.7% 5.9% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 1914 6138 191 959 6693 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 CV (%) 4.2% 7% 5.7% 5.9% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 1914 6138 191 959 6693 Mean (pg/ml) 210 1914 6138 191 959 6693 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 Standard deviation 8.82 66.78 349.86 11.26 74.8 468.51 CV (%) 4.2% 7% 5.7% 5.9% 7.8% 7% CV (%) 4.2% 7% 5.7% 5.9% 7.8% 7%

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.107 0.2 0.373 0.694 1.149 1.721 2.026 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.107 0.2 0.373 0.694 1.149 1.721 2.026 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.107 0.2 0.373 0.694 1.149 1.721 2.026 O.D. 0.012 0.107 0.2 0.373 0.694 1.149 1.721 2.026

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.