Human HGFA ELISA Kit PicoKine®

Human HGFA ELISA Kit PicoKine® (96 Tests) . Quantitate Human HGFAC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hepatocyte growth factor activator, also known as HGFA, is a protein that in humans is encoded by the HGFAC gene. This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 62 64 63 62 0.82 1.3% Sample 2 304 296 265 269 283 16.8 5.9% Sample 3 830 851 870 915 866 31.37 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 62 64 63 62 0.82 1.3% Sample 1 62 62 64 63 62 0.82 1.3% Sample 2 304 296 265 269 283 16.8 5.9% Sample 2 304 296 265 269 283 16.8 5.9% Sample 3 830 851 870 915 866 31.37 3.6% Sample 3 830 851 870 915 866 31.37 3.6%

Synonyms

Hepatocyte growth factor activator; HGF activator; HGFA;3.4.21.-; Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain; HGFAC

Gene Name

HGFAC

Gene ID

3083

UniProt

Q04756

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q36-S655

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Liver.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 304 830 60 296 836 Standard deviation 3.1 20.06 52.29 3.9 22.79 59.35 CV (%) 5% 6.6% 7.7%% 6.5% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 304 830 60 296 836 Standard deviation 3.1 20.06 52.29 3.9 22.79 59.35 CV (%) 5% 6.6% 7.7%% 6.5% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 304 830 60 296 836 Mean (pg/ml) 62 304 830 60 296 836 Standard deviation 3.1 20.06 52.29 3.9 22.79 59.35 Standard deviation 3.1 20.06 52.29 3.9 22.79 59.35 CV (%) 5% 6.6% 7.7%% 6.5% 7.7% 7.1% CV (%) 5% 6.6% 7.7%% 6.5% 7.7% 7.1%

Sensitivity

<10 pg/mL

Function

Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

70682 MW

Specificity

Natural and recombinant human HGFA

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Hepatocyte growth factor activator

Subcellular Location

Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.

Protein Name

Hepatocyte growth factor activator

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.074 0.121 0.198 0.354 0.6252 1.02 1.813 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.074 0.121 0.198 0.354 0.6252 1.02 1.813 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.074 0.121 0.198 0.354 0.6252 1.02 1.813 O.D. 0.019 0.074 0.121 0.198 0.354 0.6252 1.02 1.813

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.