Human Sclerostin/SOST ELISA Kit PicoKine®

Human Sclerostin/SOST ELISA Kit PicoKine® (96 Tests) . Quantitate Human SOST in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 84 85 97 88 5.11 5.8% Sample 2 215 183 209 209 204 12.36 6% Sample 3 883 875 1058 953 942 73.39 7.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 84 85 97 88 5.11 5.8% Sample 1 89 84 85 97 88 5.11 5.8% Sample 2 215 183 209 209 204 12.36 6% Sample 2 215 183 209 209 204 12.36 6% Sample 3 883 875 1058 953 942 73.39 7.7% Sample 3 883 875 1058 953 942 73.39 7.7%

Synonyms

Sclerostin; SOST; UNQ2976/PRO7455/PRO7476

Gene Name

SOST

Gene ID

50964

UniProt

Q9BQB4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y213

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level) . .

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Metabolism, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Skeletal Development, Stem Cells, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 215 883 90 209 912 Mean (pg/ml) 89 215 883 90 209 912 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 Standard deviation 6.75 8.6 59.16 6.74 10.65 70.22 CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7% CV (%) 6.7% 4% 6.7% 6.6% 7.7% 7.7%

Sensitivity

<10 pg/mL

Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24031 MW

Specificity

Natural and recombinant human Sclerostin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Sclerostin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Protein Name

Sclerostin

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835 O.D. 0.017 0.077 0.126 0.209 0.34 0.571 0.993 1.835

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.