Mouse CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

Mouse CA9/Ca Ix ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ca9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 35 40 34 35 2.48 7% Sample 2 186 181 178 165 177 7.76 4.3% Sample 3 448 417 503 475 460 31.87 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 35 40 34 35 2.48 7% Sample 1 34 35 40 34 35 2.48 7% Sample 2 186 181 178 165 177 7.76 4.3% Sample 2 186 181 178 165 177 7.76 4.3% Sample 3 448 417 503 475 460 31.87 6.9% Sample 3 448 417 503 475 460 31.87 6.9%

Synonyms

Carbonic anhydrase 9;4.2.1.1; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN homolog; Ca9; Car9

Gene Name

Ca9

Gene ID

230099

UniProt

Q8VHB5

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-D390

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Immunology, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 186 448 35 180 461 Standard deviation 2.55 12.27 28.67 2.73 13.32 35.49 CV (%) 7.5% 6.6% 6.4% 7.8% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 186 448 35 180 461 Standard deviation 2.55 12.27 28.67 2.73 13.32 35.49 CV (%) 7.5% 6.6% 6.4% 7.8% 7.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 186 448 35 180 461 Mean (pg/ml) 34 186 448 35 180 461 Standard deviation 2.55 12.27 28.67 2.73 13.32 35.49 Standard deviation 2.55 12.27 28.67 2.73 13.32 35.49 CV (%) 7.5% 6.6% 6.4% 7.8% 7.4% 7.7% CV (%) 7.5% 6.6% 6.4% 7.8% 7.4% 7.7%

Sensitivity

<10 pg/mL

Function

Reversible hydration of carbon dioxide. Participates in pH regulation (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47265 MW

Specificity

Natural and recombinant mouse CA9

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Carbonic anhydrase 9

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein .

Protein Name

Carbonic anhydrase 9

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.115 0.176 0.285 0.476 0.849 1.549 2.374 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.115 0.176 0.285 0.476 0.849 1.549 2.374 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.115 0.176 0.285 0.476 0.849 1.549 2.374 O.D. 0.053 0.115 0.176 0.285 0.476 0.849 1.549 2.374

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.