Mouse KLK1/Kallikrein 1 ELISA Kit PicoKine®

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 154 170 150 156 8.24 5.2% Sample 2 602 649 645 637 633 18.55 2.9% Sample 3 1476 1672 1722 1744 1653 105.74 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 154 170 150 156 8.24 5.2% Sample 1 150 154 170 150 156 8.24 5.2% Sample 2 602 649 645 637 633 18.55 2.9% Sample 2 602 649 645 637 633 18.55 2.9% Sample 3 1476 1672 1722 1744 1653 105.74 6.3% Sample 3 1476 1672 1722 1744 1653 105.74 6.3%

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 602 1476 154 580 1608 Mean (pg/ml) 150 602 1476 154 580 1608 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 Standard deviation 9.15 43.94 116.6 11.08 51.62 136.68 CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5% CV (%) 6.1% 7.3% 7.9% 7.2% 8.9% 8.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069 O.D. 0.007 0.122 0.184 0.309 0.529 0.881 1.455 2.069

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.