Human Siglec5/Cd170 ELISA Kit PicoKine®

Sialic acid-binding Ig-like lectin 5 is a protein that in humans is encoded by the SIGLEC5 gene. This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig) -like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 103 103 92 97 5.26 5.4% Sample 2 282 301 334 293 302 19.39 6.4% Sample 3 1180 1269 1248 1310 1251 47.04 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 103 103 92 97 5.26 5.4% Sample 1 93 103 103 92 97 5.26 5.4% Sample 2 282 301 334 293 302 19.39 6.4% Sample 2 282 301 334 293 302 19.39 6.4% Sample 3 1180 1269 1248 1310 1251 47.04 3.7% Sample 3 1180 1269 1248 1310 1251 47.04 3.7%

Sialic acid-binding Ig-like lectin 5; Siglec-5; CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD170; SIGLEC5; CD33L2, OBBP2

Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 282 1180 102 277 1084 Standard deviation 4.46 21.71 51.92 7.7%1 23.26 57.45 CV (%) 4.8% 7.7% 4.4% 5.5% 8.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 282 1180 102 277 1084 Standard deviation 4.46 21.71 51.92 7.7%1 23.26 57.45 CV (%) 4.8% 7.7% 4.4% 5.5% 8.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 282 1180 102 277 1084 Mean (pg/ml) 93 282 1180 102 277 1084 Standard deviation 4.46 21.71 51.92 7.7%1 23.26 57.45 Standard deviation 4.46 21.71 51.92 7.7%1 23.26 57.45 CV (%) 4.8% 7.7% 4.4% 5.5% 8.4% 5.3% CV (%) 4.8% 7.7% 4.4% 5.5% 8.4% 5.3%

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.165 0.201 0.296 0.475 0.736 1.236 1.89 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.165 0.201 0.296 0.475 0.736 1.236 1.89 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.165 0.201 0.296 0.475 0.736 1.236 1.89 O.D. 0.052 0.165 0.201 0.296 0.475 0.736 1.236 1.89

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.