Rat TNFRSF19/TROY ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 158 157 156 158 1.87 1.1% Sample 2 625 573 633 566 599 29.98 5% Sample 3 1725 1554 1742 1569 1647 86.37 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 158 157 156 158 1.87 1.1% Sample 1 161 158 157 156 158 1.87 1.1% Sample 2 625 573 633 566 599 29.98 5% Sample 2 625 573 633 566 599 29.98 5% Sample 3 1725 1554 1742 1569 1647 86.37 5.2% Sample 3 1725 1554 1742 1569 1647 86.37 5.2%

Apoptosis, Associated Proteins, Cancer, Cell Biology, Growth Factors, Growth Factors/Hormones, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 625 1725 161 663 1839 Standard deviation 11.75 33.12 115.57 13.84 43.09 156.5%1 CV (%) 7.3% 5.3% 6.7% 8.6% 6.5% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 625 1725 161 663 1839 Standard deviation 11.75 33.12 115.57 13.84 43.09 156.5%1 CV (%) 7.3% 5.3% 6.7% 8.6% 6.5% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 161 625 1725 161 663 1839 Mean (pg/ml) 161 625 1725 161 663 1839 Standard deviation 11.75 33.12 115.57 13.84 43.09 156.5%1 Standard deviation 11.75 33.12 115.57 13.84 43.09 156.5%1 CV (%) 7.3% 5.3% 6.7% 8.6% 6.5% 8.5% CV (%) 7.3% 5.3% 6.7% 8.6% 6.5% 8.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.117 0.177 0.274 0.497 0.84 1.497 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.117 0.177 0.274 0.497 0.84 1.497 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.117 0.177 0.274 0.497 0.84 1.497 2.174 O.D. 0.063 0.117 0.177 0.274 0.497 0.84 1.497 2.174

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.