Mouse TNFRSF19/TROY ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 85 77 81 81 2.95 3.6% Sample 2 466 515 454 448 470 26.35 5.6% Sample 3 1265 1257 1295 1148 1241 55.67 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 85 77 81 81 2.95 3.6% Sample 1 83 85 77 81 81 2.95 3.6% Sample 2 466 515 454 448 470 26.35 5.6% Sample 2 466 515 454 448 470 26.35 5.6% Sample 3 1265 1257 1295 1148 1241 55.67 4.4% Sample 3 1265 1257 1295 1148 1241 55.67 4.4%

Highly expressed in adult brain, and in embryos from day 11-17, but not earlier. Detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, mainly in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. Isoform 3 was found in embryonic day 17.5 skin but not in brain and liver.

Apoptosis, Associated Proteins, Cancer, Cell Biology, Growth Factors, Growth Factors/Hormones, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 466 1265 90 483 1262 Standard deviation 5.89 25.53 63.25 7.2 29.46 70.67 CV (%) 7.1% 5.5% 5% 8% 6.1% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 466 1265 90 483 1262 Standard deviation 5.89 25.53 63.25 7.2 29.46 70.67 CV (%) 7.1% 5.5% 5% 8% 6.1% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 466 1265 90 483 1262 Mean (pg/ml) 83 466 1265 90 483 1262 Standard deviation 5.89 25.53 63.25 7.2 29.46 70.67 Standard deviation 5.89 25.53 63.25 7.2 29.46 70.67 CV (%) 7.1% 5.5% 5% 8% 6.1% 5.6% CV (%) 7.1% 5.5% 5% 8% 6.1% 5.6%

Can mediate activation of c-Jun and NF-kappa-B. May promote caspase-independent cell death (By similarity) . Isoform 2 and isoform 3 may act as decoy receptors. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.054 0.103 0.173 0.304 0.52 0.973 1.53 2.043 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.054 0.103 0.173 0.304 0.52 0.973 1.53 2.043 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.054 0.103 0.173 0.304 0.52 0.973 1.53 2.043 O.D. 0.054 0.103 0.173 0.304 0.52 0.973 1.53 2.043

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.