Human TNFRSF19/TROY ELISA Kit PicoKine®

Human TNFRSF19/TROY ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 184 205 190 190 192 7.75 4% Sample 2 541 552 500 518 527 20.17 3.8% Sample 3 1334 1468 1351 1411 1391 52.86 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 184 205 190 190 192 7.75 4% Sample 1 184 205 190 190 192 7.75 4% Sample 2 541 552 500 518 527 20.17 3.8% Sample 2 541 552 500 518 527 20.17 3.8% Sample 3 1334 1468 1351 1411 1391 52.86 3.8% Sample 3 1334 1468 1351 1411 1391 52.86 3.8%

Synonyms

Tumor necrosis factor receptor superfamily member 19; TRADE; Toxicity and JNK inducer; TNFRSF19; TAJ, TROY; UNQ1888/PRO4333

Gene Name

TNFRSF19

Gene ID

55504

UniProt

Q9NS68

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E30-L170

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.

Applications

ELISA

Field of Research

Apoptosis, Associated Proteins, Cancer, Cell Biology, Growth Factors, Growth Factors/Hormones, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 184 541 1334 198 536 1337 Standard deviation 13.43 36.24 88.04 16.83 43.95 112.3 CV (%) 7.3% 6.7% 6.6% 8.5% 8.2% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 184 541 1334 198 536 1337 Standard deviation 13.43 36.24 88.04 16.83 43.95 112.3 CV (%) 7.3% 6.7% 6.6% 8.5% 8.2% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 184 541 1334 198 536 1337 Mean (pg/ml) 184 541 1334 198 536 1337 Standard deviation 13.43 36.24 88.04 16.83 43.95 112.3 Standard deviation 13.43 36.24 88.04 16.83 43.95 112.3 CV (%) 7.3% 6.7% 6.6% 8.5% 8.2% 8.4% CV (%) 7.3% 6.7% 6.6% 8.5% 8.2% 8.4%

Sensitivity

<12 pg/mL

Function

Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

46015 MW

Specificity

Natural and recombinant human TNFRSF19

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 19

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Tumor necrosis factor receptor superfamily member 19

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.048 0.137 0.239 0.405 0.669 1.138 1.654 1.936 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.048 0.137 0.239 0.405 0.669 1.138 1.654 1.936 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.048 0.137 0.239 0.405 0.669 1.138 1.654 1.936 O.D. 0.048 0.137 0.239 0.405 0.669 1.138 1.654 1.936

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.