Human Klotho beta/KLB ELISA Kit PicoKine®

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. It is mapped to chromosome 4. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) . Klotho beta is also an essential cofactor for FGF21 activity and the Klotho gene family may have evolved to confer tissue-specific bioactivity on FGF19 subfamily members.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 277 256 255 290 269 14.73 5.4% Sample . 2 1292 1377 1293 1260 1305 43.36 3.3%% Sample 3 4214 3960 4302 3984 4115 146.59 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 277 256 255 290 269 14.73 5.4% Sample 1 277 256 255 290 269 14.73 5.4% Sample . 2 1292 1377 1293 1260 1305 43.36 3.3%% Sample . 2 1292 1377 1293 1260 1305 43.36 3.3%% Sample 3 4214 3960 4302 3984 4115 146.59 3.5% Sample 3 4214 3960 4302 3984 4115 146.59 3.5%

Cancer, Cardiovascular, Cholesterol Metabolism, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Transcription

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 277 1292 4214 298 1303 4659 Standard deviation 16.9 85.27 227.56 12.8 62.5 349.4 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 277 1292 4214 298 1303 4659 Standard deviation 16.9 85.27 227.56 12.8 62.5 349.4 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 277 1292 4214 298 1303 4659 Mean (pg/ml) 277 1292 4214 298 1303 4659 Standard deviation 16.9 85.27 227.56 12.8 62.5 349.4 Standard deviation 16.9 85.27 227.56 12.8 62.5 349.4 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5%

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.121 0.22 0.391 0.671 1.143 1.688 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.121 0.22 0.391 0.671 1.143 1.688 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.121 0.22 0.391 0.671 1.143 1.688 2.051 O.D. 0.035 0.121 0.22 0.391 0.671 1.143 1.688 2.051

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.