Human TNFRSF25/DR3 ELISA Kit PicoKine®

Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 691 639 639 669 659 21.92 3.3% Sample 2 2487 2310 2253 2294 2336 89.62 3.8% Sample 3 9783 10046 9881 10103 9953 127.7 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 691 639 639 669 659 21.92 3.3% Sample 1 691 639 639 669 659 21.92 3.3% Sample 2 2487 2310 2253 2294 2336 89.62 3.8% Sample 2 2487 2310 2253 2294 2336 89.62 3.8% Sample 3 9783 10046 9881 10103 9953 127.7 1.2% Sample 3 9783 10046 9881 10103 9953 127.7 1.2%

Tumor necrosis factor receptor superfamily member 25; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; Lymphocyte-associated receptor of death; LARD; Protein WSL; Protein WSL-1; TNFRSF25; APO3, DDR3, DR3, TNFRSF12, WSL, WSL1; UNQ455/PRO779

Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 691 2487 9783 668 2430 10603 Standard deviation 37.31 154.19 489.15 42.75 196.83 689.19 CV (%) 5.4% 6.2% 5% 6.4% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 691 2487 9783 668 2430 10603 Standard deviation 37.31 154.19 489.15 42.75 196.83 689.19 CV (%) 5.4% 6.2% 5% 6.4% 8.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 691 2487 9783 668 2430 10603 Mean (pg/ml) 691 2487 9783 668 2430 10603 Standard deviation 37.31 154.19 489.15 42.75 196.83 689.19 Standard deviation 37.31 154.19 489.15 42.75 196.83 689.19 CV (%) 5.4% 6.2% 5% 6.4% 8.1% 6.5% CV (%) 5.4% 6.2% 5% 6.4% 8.1% 6.5%

Receptor for TNFSF12/APO3L/TWEAK. Interacts ly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.084 0.146 0.263 0.501 0.888 1.54 2.016 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.084 0.146 0.263 0.501 0.888 1.54 2.016 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.084 0.146 0.263 0.501 0.888 1.54 2.016 O.D. 0.008 0.084 0.146 0.263 0.501 0.888 1.54 2.016

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.