Human Afamin ELISA Kit PicoKine®

Human Afamin ELISA Kit PicoKine® (96 Tests) . Quantitate Human AFM in cell culture supernatants, serum and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Sample 3 3779 3418 3836 3710 3685 160.89 4.3%

Synonyms

Afamin; Alpha-albumin; Alpha-Alb; AFM; ALB2, ALBA

Gene Name

AFM

Gene ID

173

UniProt

P43652

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L22-N599

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level) . .

Applications

ELISA

Field of Research

Cardiovascular, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8%

Sensitivity

<10 pg/mL

Function

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

69069 MW

Specificity

Natural and recombinant human Afamin

Gene Name Synonym

Afamin

Subcellular Location

Secreted.

Protein Name

Afamin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.