Human Afamin ELISA Kit PicoKine®

Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 221 236 257 237 237 12.79 5.3% Sample 1 221 236 257 237 237 12.79 5.3% Sample 2 658 655 603 647 640 22.16 3.4% Sample 2 658 655 603 647 640 22.16 3.4% Sample 3 3779 3418 3836 3710 3685 160.89 4.3% Sample 3 3779 3418 3836 3710 3685 160.89 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 221 658 3779 249 672 4128 Mean (pg/ml) 221 658 3779 249 672 4128 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 Standard deviation 10.83 33.56 219.18 17.68 51.07 321.98 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8%

Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.