Human FOLR2/Beta Fr ELISA Kit PicoKine®

Human FOLR2/Beta Fr ELISA Kit PicoKine® (96 Tests) . Quantitate Human FOLR2 in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Folate receptor beta is a protein that in humans is encoded by the FOLR2 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 342 322 342 345 337 9.17 2.7% Sample 2 1852 1882 1780 2001 1878 79.72 4.2% Sample 3 4326 4126 3772 4098 4080 198.63 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 342 322 342 345 337 9.17 2.7% Sample 1 342 322 342 345 337 9.17 2.7% Sample 2 1852 1882 1780 2001 1878 79.72 4.2% Sample 2 1852 1882 1780 2001 1878 79.72 4.2% Sample 3 4326 4126 3772 4098 4080 198.63 4.8% Sample 3 4326 4126 3772 4098 4080 198.63 4.8%

Synonyms

Folate receptor beta; FR-beta; Folate receptor 2; Folate receptor, fetal/placental; Placental folate-binding protein; FBP; FOLR2

Gene Name

FOLR2

Gene ID

2350

UniProt

P14207

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T17-N230

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in placenta and hematopoietic cells. Expression is increased in malignant tissues. .

Applications

ELISA

Field of Research

Cancer, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 1852 4326 361 1834 4010 Standard deviation 23.25 85.49 190.34 29.96 102.7 216.54 CV (%) 6.8% 4.6% 4.4% 8.3% 5.6% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 1852 4326 361 1834 4010 Standard deviation 23.25 85.49 190.34 29.96 102.7 216.54 CV (%) 6.8% 4.6% 4.4% 8.3% 5.6% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 342 1852 4326 361 1834 4010 Mean (pg/ml) 342 1852 4326 361 1834 4010 Standard deviation 23.25 85.49 190.34 29.96 102.7 216.54 Standard deviation 23.25 85.49 190.34 29.96 102.7 216.54 CV (%) 6.8% 4.6% 4.4% 8.3% 5.6% 5.4% CV (%) 6.8% 4.6% 4.4% 8.3% 5.6% 5.4%

Sensitivity

<1 pg/mL

Function

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

29280 MW

Specificity

Natural and recombinant human FOLR2

Gene Name Synonym

Folate receptor beta

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Secreted .

Protein Name

Folate receptor beta

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.09 0.129 0.193 0.328 0.571 0.975 1.792 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.09 0.129 0.193 0.328 0.571 0.975 1.792 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.09 0.129 0.193 0.328 0.571 0.975 1.792 O.D. 0.057 0.09 0.129 0.193 0.328 0.571 0.975 1.792

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.