Human Serpin A7 ELISA Kit PicoKine®

Thyroxine-binding globulin (TBG), also known as SERPINA7, is the major thyroid hormone transport protein in serum. There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. Using DNA blot hybridization, it is detected that there is a single-copy TBG gene located on the long arm of the X chromosome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 87 98 94 90 6.51 7.2% Sample 2 314 310 300 278 300 13.95 4.6% Sample 3 875 1044 945 949 953 60.09 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 87 98 94 90 6.51 7.2% Sample 1 81 87 98 94 90 6.51 7.2% Sample 2 314 310 300 278 300 13.95 4.6% Sample 2 314 310 300 278 300 13.95 4.6% Sample 3 875 1044 945 949 953 60.09 6.3% Sample 3 875 1044 945 949 953 60.09 6.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 314 875 75 294 845 Standard deviation 4.94 16.64 45.5 5.47 15.87 49.01 CV (%) 6.1% 5.3% 5.2% 7.3% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 314 875 75 294 845 Standard deviation 4.94 16.64 45.5 5.47 15.87 49.01 CV (%) 6.1% 5.3% 5.2% 7.3% 5.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 314 875 75 294 845 Mean (pg/ml) 81 314 875 75 294 845 Standard deviation 4.94 16.64 45.5 5.47 15.87 49.01 Standard deviation 4.94 16.64 45.5 5.47 15.87 49.01 CV (%) 6.1% 5.3% 5.2% 7.3% 5.4% 5.8% CV (%) 6.1% 5.3% 5.2% 7.3% 5.4% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.11 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.11 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.11 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.11

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.