Mouse DTK/TYRO3 ELISA Kit PicoKine®

Tyrosine-protein kinase receptor TYRO3 is an enzyme that in humans is encoded by the TYRO3 gene. The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 189 193 182 182 9.9 5.4% Sample 2 1343 1576 1399 1469 1446 86.95 6% Sample 3 5143 5321 4937 5555 5239 227.48 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 167 189 193 182 182 9.9 5.4% Sample 1 167 189 193 182 182 9.9 5.4% Sample 2 1343 1576 1399 1469 1446 86.95 6% Sample 2 1343 1576 1399 1469 1446 86.95 6% Sample 3 5143 5321 4937 5555 5239 227.48 4.3% Sample 3 5143 5321 4937 5555 5239 227.48 4.3%

Tyrosine-protein kinase receptor TYRO3;2.7.10.1; Etk2/tyro3; TK19-2; Tyrosine-protein kinase DTK; Tyrosine-protein kinase RSE; Tyrosine-protein kinase TIF; Tyro3; Dtk, Rse, Tif

Cell Adhesion Proteins, Intracellular Signaling, Kinases, Membrane Proteins, Neuroscience, Neurotransmission, Protein Phosphorylation, Signal Transduction, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 1343 5143 169 1385 5492 Standard deviation 12.19 96.69 324 12.33 114.95 384.44 CV (%) 7.3% 7.2% 8.3%% 7.3% 8.3% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 167 1343 5143 169 1385 5492 Standard deviation 12.19 96.69 324 12.33 114.95 384.44 CV (%) 7.3% 7.2% 8.3%% 7.3% 8.3% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 167 1343 5143 169 1385 5492 Mean (pg/ml) 167 1343 5143 169 1385 5492 Standard deviation 12.19 96.69 324 12.33 114.95 384.44 Standard deviation 12.19 96.69 324 12.33 114.95 384.44 CV (%) 7.3% 7.2% 8.3%% 7.3% 8.3% 7% CV (%) 7.3% 7.2% 8.3%% 7.3% 8.3% 7%

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259 O.D. 0.015 0.088 0.156 0.243 0.502 0.898 1.605 2.259

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.