Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine®

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine® (96 Tests) . Quantitate Human A1BG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 256 258 231 245 11.9 4.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 3 5607 5964 6206 6301 6019 267.98 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 256 258 231 245 11.9 4.8% Sample 1 236 256 258 231 245 11.9 4.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 2 1951 1726 1646 1670 1748 120.6 6.8% Sample 3 5607 5964 6206 6301 6019 267.98 4.4% Sample 3 5607 5964 6206 6301 6019 267.98 4.4%

Synonyms

Alpha-1B-glycoprotein; Alpha-1-B glycoprotein; A1BG

Gene Name

A1BG

Gene ID

UniProt

P04217

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-S495

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Plasma.

Applications

ELISA

Field of Research

Cell Biology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1951 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 495.9 CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1951 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 495.9 CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 1951 5607 256 1869 5510 Mean (pg/ml) 236 1951 5607 256 1869 5510 Standard deviation 15.81 85.84 409.31 20.48 89.71 495.9 Standard deviation 15.81 85.84 409.31 20.48 89.71 495.9 CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9% CV (%) 6.7% 4.4% 7.3% 8% 4.8% 9%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

54254 MW

Specificity

Natural and recombinant human A1BG

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-1B-glycoprotein

Subcellular Location

Secreted.

Protein Name

Alpha-1B-glycoprotein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.069 0.114 0.217 0.395 0.747 1.332 1.901 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.069 0.114 0.217 0.395 0.747 1.332 1.901 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.069 0.114 0.217 0.395 0.747 1.332 1.901 O.D. 0.031 0.069 0.114 0.217 0.395 0.747 1.332 1.901

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.