Monkey primate SFRP5/Sarp3 ELISA Kit PicoKine®

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 353 327 347 339 11.22 3.3% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 3 4969 5003 5645 5635 5313 327.23 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 353 327 347 339 11.22 3.3% Sample 1 329 353 327 347 339 11.22 3.3% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 3 4969 5003 5645 5635 5313 327.23 6.1% Sample 3 4969 5003 5645 5635 5313 327.23 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8%

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.035 0.06 0.12 0.278 0.681 1.443 2.018 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.035 0.06 0.12 0.278 0.681 1.443 2.018 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.035 0.06 0.12 0.278 0.681 1.443 2.018 O.D. 0.006 0.035 0.06 0.12 0.278 0.681 1.443 2.018

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.