Monkey primate TNFSF13/APRIL ELISA Kit PicoKine®

Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256) . The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 265 272 285 271 8.38 3% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 3 4235 4377 4466 4871 4487 236.37 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 265 272 285 271 8.38 3% Sample 1 264 265 272 285 271 8.38 3% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 2 1700 1548 1563 1481 1573 79.55 5% Sample 3 4235 4377 4466 4871 4487 236.37 5.2% Sample 3 4235 4377 4466 4871 4487 236.37 5.2%

Adaptive Immunity, B Cells, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Non-CD, Signal Transduction, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 1700 4235 241 1784 4408 Mean (pg/ml) 264 1700 4235 241 1784 4408 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 Standard deviation 14.25 119 237.16 15.42 144.5 317.37 CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2% CV (%) 5.4% 7% 5.6% 6.4% 8.1% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.129 0.196 0.345 0.663 1.141 1.909 2.511 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.129 0.196 0.345 0.663 1.141 1.909 2.511 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.129 0.196 0.345 0.663 1.141 1.909 2.511 O.D. 0.045 0.129 0.196 0.345 0.663 1.141 1.909 2.511

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.