Monkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to monkey primate 15q15 and the cognate gene to monkey primate chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Invasion/Microenvironment, Metabolism, Metabolism Processes, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 15.5 32 52 16.5 38 64 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 CV (%) 6 5.5 4 7.8 6.5 7.2 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (ng/ml) 15.5 32 52 16.5 38 64 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 CV (%) 6 5.5 4 7.8 6.5 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (ng/ml) 15.5 32 52 16.5 38 64 Mean (ng/ml) 15.5 32 52 16.5 38 64 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 Standard deviation 0.93 1.76 2.08 1.27 2.47 4.61 CV (%) 6 5.5 4 7.8 6.5 7.2 CV (%) 6 5.5 4 7.8 6.5 7.2

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity) . Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.0 0.098 0.152 0.256 0.424 0.778 1.472 2.457 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.0 0.098 0.152 0.256 0.424 0.778 1.472 2.457 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.0 0.098 0.152 0.256 0.424 0.778 1.472 2.457 O.D. 0.0 0.098 0.152 0.256 0.424 0.778 1.472 2.457

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.