Monkey primate TIMP-3 ELISA Kit PicoKine®

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Sample 3 4383 3655 4220 4320 4144 288.52 6.9%

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Signal Transduction, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1%

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.102 0.19 0.348 0.634 1.069 1.631 2.217 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.102 0.19 0.348 0.634 1.069 1.631 2.217 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.102 0.19 0.348 0.634 1.069 1.631 2.217 O.D. 0.025 0.102 0.19 0.348 0.634 1.069 1.631 2.217

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.