Rabbit SFRP5 ELISA Kit PicoKine®

Rabbit SFRP5 ELISA Kit PicoKine® (96 Tests) . Quantitate Rabbit SFRP5 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 353 327 347 339 11.22 3.3% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 3 4969 5003 5645 5635 5313 327.23 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 353 327 347 339 11.22 3.3% Sample 1 329 353 327 347 339 11.22 3.3% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 2 1068 1216 1033 1211 1132 82.45 7.2% Sample 3 4969 5003 5645 5635 5313 327.23 6.1% Sample 3 4969 5003 5645 5635 5313 327.23 6.1%

Synonyms

Secreted frizzled-related protein 5; sFRP-5; Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3; SFRP5; FRP1B, SARP3

Gene Name

SFRP5

Gene ID

100355147

UniProt

Q5T4F7

Reactivity

Rabbit

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E30-H317

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from sheep

Tissue Specificity

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle. .

Applications

ELISA

Field of Research

Secreted, Signaling Pathways, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 329 1068 4969 309 1159 5218 Mean (pg/ml) 329 1068 4969 309 1159 5218 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 Standard deviation 20.39 69.42 203.72 21.93 96.19 250.46 CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8% CV (%) 6.2% 6.5% 4.1% 7.1% 8.3% 4.8%

Sensitivity

<15 pg/mL

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35563 MW

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Secreted frizzled-related protein 5

Subcellular Location

Secreted .

Protein Name

Secreted frizzled-related protein 5

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.041 0.066 0.126 0.284 0.687 1.449 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.041 0.066 0.126 0.284 0.687 1.449 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.041 0.066 0.126 0.284 0.687 1.449 2.024 O.D. 0.012 0.041 0.066 0.126 0.284 0.687 1.449 2.024

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.