Rabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 193 211 223 212 12.27 5.7% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 3 4928 5617 4816 5488 5212 345.56 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 193 211 223 212 12.27 5.7% Sample 1 223 193 211 223 212 12.27 5.7% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 3 4928 5617 4816 5488 5212 345.56 6.6% Sample 3 4928 5617 4816 5488 5212 345.56 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.05 0.096 0.14 0.221 0.358 0.602 1.061 1.956 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.05 0.096 0.14 0.221 0.358 0.602 1.061 1.956 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.05 0.096 0.14 0.221 0.358 0.602 1.061 1.956 O.D. 0.05 0.096 0.14 0.221 0.358 0.602 1.061 1.956

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.