Rabbit TGF Alpha ELISA Kit PicoKine®

Transforming growth factor alpha (TGF-alpha) is upregulated in some rabbit cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to rabbit chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic rabbit TGF-alpha showed similar immunoreactivity when compared with rabbit TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 225 516 126 238 509 Standard deviation 5.43 12.83 16.5 8.95 14.28 27 CV (%) 4.6 5.7 3.2 7.1 6 5.3 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 225 516 126 238 509 Standard deviation 5.43 12.83 16.5 8.95 14.28 27 CV (%) 4.6 5.7 3.2 7.1 6 5.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 118 225 516 126 238 509 Mean (pg/ml) 118 225 516 126 238 509 Standard deviation 5.43 12.83 16.5 8.95 14.28 27 Standard deviation 5.43 12.83 16.5 8.95 14.28 27 CV (%) 4.6 5.7 3.2 7.1 6 5.3 CV (%) 4.6 5.7 3.2 7.1 6 5.3

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.115 0.196 0.347 0.61 0.981 1.635 2.072 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.115 0.196 0.347 0.61 0.981 1.635 2.072 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.115 0.196 0.347 0.61 0.981 1.635 2.072 O.D. 0.03 0.115 0.196 0.347 0.61 0.981 1.635 2.072

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.