Chinese Hamster IGF-2 ELISA Kit PicoKine®

Chinese Hamster IGF-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Chinese Igf2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Synonyms

Insulin-like growth factor II; IGF-II; Multiplication-stimulating activity; MSA; Multiplication-stimulating polypeptide; Insulin-like growth factor II; Preptin; Igf2; Igf-2

Gene Name

Igf2

Gene ID

100762359

UniProt

P01346

Reactivity

Hamster

Cross Reactivity

There is no detectable cross-reactivity with IGF-1.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-E91

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Developmental Biology, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 478 1536 2743 453 1737 2968 Standard deviation 20.55 87.55 126.2 30.8 140.7 222.6 CV (%) 4.3 5.7 4.6 6.8 8.1 7.5 Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 478 1536 2743 453 1737 2968 Standard deviation 20.55 87.55 126.2 30.8 140.7 222.6 CV (%) 4.3 5.7 4.6 6.8 8.1 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 478 1536 2743 453 1737 2968 Mean (pg/ml) 478 1536 2743 453 1737 2968 Standard deviation 20.55 87.55 126.2 30.8 140.7 222.6 Standard deviation 20.55 87.55 126.2 30.8 140.7 222.6 CV (%) 4.3 5.7 4.6 6.8 8.1 7.5 CV (%) 4.3 5.7 4.6 6.8 8.1 7.5

Sensitivity

<5 pg/mL

Function

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20086 MW

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor II

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the insulin family.

Protein Name

Insulin-like growth factor II

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171 O.D. 0.049 0.112 0.196 0.309 0.593 1.04 1.668 2.171

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.