Bovine Endothelin ELISA Kit PicoKine®

Bovine Endothelin ELISA Kit PicoKine® (96 Tests) . Quantitate Bovine Endothelin in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Bovine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Synonyms

Endothelin-1; Preproendothelin-1; PPET1; Endothelin-1; ET-1; Big endothelin-1; EDN1

Gene Name

Endothelin

Gene ID

281137, 319094, 513753

UniProt

P17322, Q867A9, A6QLQ7

Reactivity

Bovine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

A peptide reflecting the target protein in bovine. Sequence: C53-W73

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

Applications

ELISA

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

3.9 pg/mL - 250 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.1 85.4 146 23.2 84.3 143 Standard deviation 1.41 3.76 8.03 1.79 6.07 8.72 CV (%) 4.4% 4.4% 5.5% 7.7% 7.2% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 26.1 85.4 146 23.2 84.3 143 Standard deviation 1.41 3.76 8.03 1.79 6.07 8.72 CV (%) 4.4% 4.4% 5.5% 7.7% 7.2% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 26.1 85.4 146 23.2 84.3 143 Mean (pg/ml) 26.1 85.4 146 23.2 84.3 143 Standard deviation 1.41 3.76 8.03 1.79 6.07 8.72 Standard deviation 1.41 3.76 8.03 1.79 6.07 8.72 CV (%) 4.4% 4.4% 5.5% 7.7% 7.2% 6.1% CV (%) 4.4% 4.4% 5.5% 7.7% 7.2% 6.1%

Sensitivity

<0.5 pg/mL

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24425 MW

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Endothelin 1, endothelin 2, endothelin 3

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the endothelin/sarafotoxin family.

Protein Name

Endothelin-1, Endothelin-2, Endothelin-3

Sample Data

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.011 0.06 0.107 0.199 0.428 0.882 1.656 2.461 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.011 0.06 0.107 0.199 0.428 0.882 1.656 2.461 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.011 0.06 0.107 0.199 0.428 0.882 1.656 2.461 O.D. 0.011 0.06 0.107 0.199 0.428 0.882 1.656 2.461

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.