Bovine IGF-1 ELISA Kit PicoKine®

Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Bovine IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A) . The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP) . Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 260 292 291 276 14.82 5.3% Sample 2 870 892 954 963 919 39.65 4.3% Sample 3 2892 2468 2606 2661 2656 152.94 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 260 292 291 276 14.82 5.3% Sample 1 264 260 292 291 276 14.82 5.3% Sample 2 870 892 954 963 919 39.65 4.3% Sample 2 870 892 954 963 919 39.65 4.3% Sample 3 2892 2468 2606 2661 2656 152.94 5.7% Sample 3 2892 2468 2606 2661 2656 152.94 5.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 870 2892 298 962 3741 Standard deviation 11.09 37.41 118.57 19.67 65.42 265.61 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 870 2892 298 962 3741 Standard deviation 11.09 37.41 118.57 19.67 65.42 265.61 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 870 2892 298 962 3741 Mean (pg/ml) 264 870 2892 298 962 3741 Standard deviation 11.09 37.41 118.57 19.67 65.42 265.61 Standard deviation 11.09 37.41 118.57 19.67 65.42 265.61 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1%

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.142 0.214 0.357 0.616 1.064 1.809 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.142 0.214 0.357 0.616 1.064 1.809 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.106 0.142 0.214 0.357 0.616 1.064 1.809 O.D. 0.056 0.106 0.142 0.214 0.357 0.616 1.064 1.809

Activate the sample Cell culture supernate: add activating reagent pro rata, i.e. add 20ul of Solution A into 100ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 20ul of Solution A into 40ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant IGF-1. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.