Dog Canine IL-29 ELISA Kit PicoKine®

Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in Dog Canineas is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 91 83 91 88 3.34 3.7% Sample 2 415 360 421 388 396 24.21 6.1% Sample 3 1566 1471 1612 1562 1552 51.12 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 91 83 91 88 3.34 3.7% Sample 1 90 91 83 91 88 3.34 3.7% Sample 2 415 360 421 388 396 24.21 6.1% Sample 2 415 360 421 388 396 24.21 6.1% Sample 3 1566 1471 1612 1562 1552 51.12 3.2% Sample 3 1566 1471 1612 1562 1552 51.12 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 415 1566 103 448 1895 Mean (pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3% CV (%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3%

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03 O.D. 0.034 0.07 0.119 0.208 0.4 0.736 1.252 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.