Human CHL1/L1CAM-2 ELISA Kit PicoKine®

Human CHL1/L1CAM-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CHL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 291 285 268 269 278 9.98 3.5% Sample 2 1965 1964 2077 2085 2022 58.31 2.8% Sample 3 5542 5306 5133 4837 5204 257.08 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 291 285 268 269 278 9.98 3.5% Sample 1 291 285 268 269 278 9.98 3.5% Sample 2 1965 1964 2077 2085 2022 58.31 2.8% Sample 2 1965 1964 2077 2085 2022 58.31 2.8% Sample 3 5542 5306 5133 4837 5204 257.08 4.9% Sample 3 5542 5306 5133 4837 5204 257.08 4.9%

Synonyms

Neural cell adhesion molecule L1-like protein; Close homolog of L1; Processed neural cell adhesion molecule L1-like protein; CHL1; CALL

Gene Name

CHL1

Gene ID

10752

UniProt

O00533

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-Q1096

Tissue Specificity

Expressed in the fetal and adult brain as well as in Schwann cell culture. Also detected in adult peripheral tissues. .

Applications

ELISA

Field of Research

Actin Binding Proteins, Actin, etc., Cell Adhesion Proteins, Cytoskeleton, Cytoskeleton/ECM, ECM Proteins, Membrane Proteins, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 291 1965 5542 267 2117 5729 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 CV (%) 7.6% 7.2% 6.2% 7.9% 9.1% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 291 1965 5542 267 2117 5729 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 CV (%) 7.6% 7.2% 6.2% 7.9% 9.1% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 291 1965 5542 267 2117 5729 Mean (pg/ml) 291 1965 5542 267 2117 5729 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 Standard deviation 22.11 141.48 343.6 21.09 192.64 435.4 CV (%) 7.6% 7.2% 6.2% 7.9% 9.1% 7.6% CV (%) 7.6% 7.2% 6.2% 7.9% 9.1% 7.6%

Sensitivity

<10 pg/mL

Function

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

135071 MW

Specificity

Natural and recombinant human CHL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neural cell adhesion molecule L1-like protein

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Soluble forms produced by cleavage/shedding also exist. .

Protein Name

Neural cell adhesion molecule L1-like protein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.075 0.141 0.273 0.504 0.914 1.41 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.075 0.141 0.273 0.504 0.914 1.41 1.912 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.028 0.075 0.141 0.273 0.504 0.914 1.41 1.912 O.D. 0.028 0.075 0.141 0.273 0.504 0.914 1.41 1.912

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.