Mouse DKK4 ELISA Kit PicoKine®

DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 115 112 113 121 115 3.49 3% Sample 2 828 710 788 755 770 43.33 5.6% Sample 3 2616 2592 2575 2681 2616 40.25 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 115 112 113 121 115 3.49 3% Sample 1 115 112 113 121 115 3.49 3% Sample 2 828 710 788 755 770 43.33 5.6% Sample 2 828 710 788 755 770 43.33 5.6% Sample 3 2616 2592 2575 2681 2616 40.25 1.5% Sample 3 2616 2592 2575 2681 2616 40.25 1.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 115 828 2616 104 829 2555 Standard deviation 7.13 36.43 133.41 7.9%1 51.39 178.85 CV (%) 6.2% 4.4% 7%% 7.9% 6.2% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 115 828 2616 104 829 2555 Standard deviation 7.13 36.43 133.41 7.9%1 51.39 178.85 CV (%) 6.2% 4.4% 7%% 7.9% 6.2% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 115 828 2616 104 829 2555 Mean (pg/ml) 115 828 2616 104 829 2555 Standard deviation 7.13 36.43 133.41 7.9%1 51.39 178.85 Standard deviation 7.13 36.43 133.41 7.9%1 51.39 178.85 CV (%) 6.2% 4.4% 7%% 7.9% 6.2% 7% CV (%) 6.2% 4.4% 7%% 7.9% 6.2% 7%

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.211 0.242 0.355 0.535 0.889 1.466 2.017 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.211 0.242 0.355 0.535 0.889 1.466 2.017 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.211 0.242 0.355 0.535 0.889 1.466 2.017 O.D. 0.031 0.211 0.242 0.355 0.535 0.889 1.466 2.017

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.