Human S100A13 ELISA Kit PicoKine®

S100 calcium-binding protein A13 (S100A13) is a protein that in humans is encoded by the S100A13 gene. The characterization of a human gene, S100A13 encodes a calcium-binding protein belonging to the S100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 38 36 38 37 0.86 2.3% Sample 2 138 140 138 152 142 5.83 4.1% Sample 3 472 455 478 440 461 14.88 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 38 36 38 37 0.86 2.3% Sample 1 38 38 36 38 37 0.86 2.3% Sample 2 138 140 138 152 142 5.83 4.1% Sample 2 138 140 138 152 142 5.83 4.1% Sample 3 472 455 478 440 461 14.88 3.2% Sample 3 472 455 478 440 461 14.88 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 138 472 38 130 466 Standard deviation 1.78 5.79 29.73 1.9 6.63 34.95 CV (%) 4.7% 4.2% 7.5%%% 5% 7.5%% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 138 472 38 130 466 Standard deviation 1.78 5.79 29.73 1.9 6.63 34.95 CV (%) 4.7% 4.2% 7.5%%% 5% 7.5%% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 138 472 38 130 466 Mean (pg/ml) 38 138 472 38 130 466 Standard deviation 1.78 5.79 29.73 1.9 6.63 34.95 Standard deviation 1.78 5.79 29.73 1.9 6.63 34.95 CV (%) 4.7% 4.2% 7.5%%% 5% 7.5%% 7.5% CV (%) 4.7% 4.2% 7.5%%% 5% 7.5%% 7.5%

Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.046 0.08 0.161 0.299 0.564 1.015 1.805 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.046 0.08 0.161 0.299 0.564 1.015 1.805 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.046 0.08 0.161 0.299 0.564 1.015 1.805 O.D. 0.003 0.046 0.08 0.161 0.299 0.564 1.015 1.805

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.