Human FABP2/I-FABP ELISA Kit PicoKine®

Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 56 59 50 53 4.15 7.8% Sample 2 270 308 313 281 293 18.01 6.1% Sample 3 1038 997 1031 1019 1021 15.56 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 56 59 50 53 4.15 7.8% Sample 1 49 56 59 50 53 4.15 7.8% Sample 2 270 308 313 281 293 18.01 6.1% Sample 2 270 308 313 281 293 18.01 6.1% Sample 3 1038 997 1031 1019 1021 15.56 1.5% Sample 3 1038 997 1031 1019 1021 15.56 1.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 270 1038 51 272 977 Standard deviation 2.69 14.31 61.24 3.57 18.76 74.25 CV (%) 5.5% 5.3% 5.9% 7% 6.9% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 270 1038 51 272 977 Standard deviation 2.69 14.31 61.24 3.57 18.76 74.25 CV (%) 5.5% 5.3% 5.9% 7% 6.9% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 270 1038 51 272 977 Mean (pg/ml) 49 270 1038 51 272 977 Standard deviation 2.69 14.31 61.24 3.57 18.76 74.25 Standard deviation 2.69 14.31 61.24 3.57 18.76 74.25 CV (%) 5.5% 5.3% 5.9% 7% 6.9% 7.6% CV (%) 5.5% 5.3% 5.9% 7% 6.9% 7.6%

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.154 0.215 0.31 0.462 0.77 1.192 1.837 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.154 0.215 0.31 0.462 0.77 1.192 1.837 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.154 0.215 0.31 0.462 0.77 1.192 1.837 O.D. 0.075 0.154 0.215 0.31 0.462 0.77 1.192 1.837

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.