Mouse CCL25/TECK ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine) . The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 92 86 94 90 2.95 3.2% Sample 2 258 283 257 304 275 19.47 7% Sample 3 916 985 981 951 958 27.7 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 92 86 94 90 2.95 3.2% Sample 1 90 92 86 94 90 2.95 3.2% Sample 2 258 283 257 304 275 19.47 7% Sample 2 258 283 257 304 275 19.47 7% Sample 3 916 985 981 951 958 27.7 2.8% Sample 3 916 985 981 951 958 27.7 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 258 916 98 271 971 Standard deviation 7.8%3 17.54 71.44 6.07 20.86 75.73 CV (%) 5.7% 6.8% 7.8% 6.2% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 258 916 98 271 971 Standard deviation 7.8%3 17.54 71.44 6.07 20.86 75.73 CV (%) 5.7% 6.8% 7.8% 6.2% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 258 916 98 271 971 Mean (pg/ml) 90 258 916 98 271 971 Standard deviation 7.8%3 17.54 71.44 6.07 20.86 75.73 Standard deviation 7.8%3 17.54 71.44 6.07 20.86 75.73 CV (%) 5.7% 6.8% 7.8% 6.2% 7.7% 7.8% CV (%) 5.7% 6.8% 7.8% 6.2% 7.7% 7.8%

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.16 0.241 0.393 0.611 1.037 1.626 2.665 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.16 0.241 0.393 0.611 1.037 1.626 2.665 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.16 0.241 0.393 0.611 1.037 1.626 2.665 O.D. 0.052 0.16 0.241 0.393 0.611 1.037 1.626 2.665

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.