Mouse Syndecan-3/SDC3 ELISA Kit PicoKine®

Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 295 333 330 288 311 20.18 6.4% Sample 2 1800 1725 1781 1910 1804 67.12 3.7% Sample 3 4508 4397 4649 3887 4360 287.45 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 295 333 330 288 311 20.18 6.4% Sample 1 295 333 330 288 311 20.18 6.4% Sample 2 1800 1725 1781 1910 1804 67.12 3.7% Sample 2 1800 1725 1781 1910 1804 67.12 3.7% Sample 3 4508 4397 4649 3887 4360 287.45 6.5% Sample 3 4508 4397 4649 3887 4360 287.45 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2280 1800 4508 280 1660 4073 Standard deviation 17.8%3 140.4 193.84 20.16 129.48 236.23 CV (%) 5.3% 7.8% 4.3% 7.2% 7.8% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2280 1800 4508 280 1660 4073 Standard deviation 17.8%3 140.4 193.84 20.16 129.48 236.23 CV (%) 5.3% 7.8% 4.3% 7.2% 7.8% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2280 1800 4508 280 1660 4073 Mean (pg/ml) 2280 1800 4508 280 1660 4073 Standard deviation 17.8%3 140.4 193.84 20.16 129.48 236.23 Standard deviation 17.8%3 140.4 193.84 20.16 129.48 236.23 CV (%) 5.3% 7.8% 4.3% 7.2% 7.8% 5.8% CV (%) 5.3% 7.8% 4.3% 7.2% 7.8% 5.8%

Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.069 0.122 0.229 0.452 0.812 1.498 2.02 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.069 0.122 0.229 0.452 0.812 1.498 2.02 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.069 0.122 0.229 0.452 0.812 1.498 2.02 O.D. 0.008 0.069 0.122 0.229 0.452 0.812 1.498 2.02

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.