Mouse Syndecan-1/SDC1 ELISA Kit PicoKine®

Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 326 374 349 330 344 18.99 5.5% Sample 2 1681 1814 1841 1691 1756 71.47 4% Sample 3 5402 5521 5466 5324 5428 73.45 1.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 326 374 349 330 344 18.99 5.5% Sample 1 326 374 349 330 344 18.99 5.5% Sample 2 1681 1814 1841 1691 1756 71.47 4% Sample 2 1681 1814 1841 1691 1756 71.47 4% Sample 3 5402 5521 5466 5324 5428 73.45 1.3% Sample 3 5402 5521 5466 5324 5428 73.45 1.3%

ECM Proteins, Extracellular Matrix, Hematopoietic Progenitors, Invasion/Microenvironment, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lymphoid, Membrane Proteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Pathways and Processes, Signal Transduction, Stem Cells, Synapse Marker

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 326 1681 5402 348 1654 5887 Standard deviation 28.10 122.71 383.54 29.92 129.01 476.84 CV (%) 7.7% 7.3% 7.1% 8.6% 7.8% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 326 1681 5402 348 1654 5887 Standard deviation 28.10 122.71 383.54 29.92 129.01 476.84 CV (%) 7.7% 7.3% 7.1% 8.6% 7.8% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 326 1681 5402 348 1654 5887 Mean (pg/ml) 326 1681 5402 348 1654 5887 Standard deviation 28.10 122.71 383.54 29.92 129.01 476.84 Standard deviation 28.10 122.71 383.54 29.92 129.01 476.84 CV (%) 7.7% 7.3% 7.1% 8.6% 7.8% 8.1% CV (%) 7.7% 7.3% 7.1% 8.6% 7.8% 8.1%

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.133 0.221 0.388 0.688 1.14 1.757 2.229 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.133 0.221 0.388 0.688 1.14 1.757 2.229 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.133 0.221 0.388 0.688 1.14 1.757 2.229 O.D. 0.008 0.133 0.221 0.388 0.688 1.14 1.757 2.229

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.