Rat GDF-15 ELISA Kit PicoKine®

Rat GDF-15 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Gdf15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GDF-15 (Growth differentiation factor 15), also known as TGF-PL, MIC-1, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 29 29 28 0.82 2.9% Sample 2 247 213 209 226 223 14.82 6.6% Sample 3 440 451 415 406 428 18.2 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 29 29 28 0.82 2.9% Sample 1 28 27 29 29 28 0.82 2.9% Sample 2 247 213 209 226 223 14.82 6.6% Sample 2 247 213 209 226 223 14.82 6.6% Sample 3 440 451 415 406 428 18.2 4.2% Sample 3 440 451 415 406 428 18.2 4.2%

Synonyms

Growth/differentiation factor 15; GDF-15; Gdf15; Sbf

Gene Name

Gdf15

Gene ID

29455

UniProt

Q9Z0J6

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S189-A303

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Hypertrophy, Signal Transduction, Structures

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 247 440 26 269 409 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 CV (%) 4.7% 4.1% 4.3% 5.3% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 247 440 26 269 409 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 CV (%) 4.7% 4.1% 4.3% 5.3% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 247 440 26 269 409 Mean (pg/ml) 28 247 440 26 269 409 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 Standard deviation 1.31 10.12 18.92 1.37 13.18 22.9 CV (%) 4.7% 4.1% 4.3% 5.3% 4.9% 5.6% CV (%) 4.7% 4.1% 4.3% 5.3% 4.9% 5.6%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

33439 MW

Specificity

Natural and recombinant rat GDF-15

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth/differentiation factor 15

Subcellular Location

Secreted .

Protein Name

Growth/differentiation factor 15

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.117 0.212 0.381 0.6257 1.064 1.476 1.917 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.117 0.212 0.381 0.6257 1.064 1.476 1.917 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.117 0.212 0.381 0.6257 1.064 1.476 1.917 O.D. 0.006 0.117 0.212 0.381 0.6257 1.064 1.476 1.917

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.