Rat CD56/NCAM-1 ELISA Kit PicoKine®

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. And the encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1208 1266 1216 1295 1246 35.86 2.8% Sample 2 4356 3976 3792 4024 4037 203.51 5% Sample 3 19046 21680 19420 19020 19791 1101.75 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1208 1266 1216 1295 1246 35.86 2.8% Sample 1 1208 1266 1216 1295 1246 35.86 2.8% Sample 2 4356 3976 3792 4024 4037 203.51 5% Sample 2 4356 3976 3792 4024 4037 203.51 5% Sample 3 19046 21680 19420 19020 19791 1101.75 5.5% Sample 3 19046 21680 19420 19020 19791 1101.75 5.5%

Axonal Guidance Proteins, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, ECM Proteins, Growth and Development, Hematopoietic Progenitors, Membrane Proteins, Nervous System Development, Neural Signal Transduction, Neurology Process, Neuron Marker, Neuroscience, Organogenesis, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1208 4356 19046 1152 4200 19885 Standard deviation 57.98 204.73 1047.53 72.57 201.6 1411.83 CV (%) 4.8% 4.7% 5.5% 4.8%% 4.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1208 4356 19046 1152 4200 19885 Standard deviation 57.98 204.73 1047.53 72.57 201.6 1411.83 CV (%) 4.8% 4.7% 5.5% 4.8%% 4.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1208 4356 19046 1152 4200 19885 Mean (pg/ml) 1208 4356 19046 1152 4200 19885 Standard deviation 57.98 204.73 1047.53 72.57 201.6 1411.83 Standard deviation 57.98 204.73 1047.53 72.57 201.6 1411.83 CV (%) 4.8% 4.7% 5.5% 4.8%% 4.8% 7.1% CV (%) 4.8% 4.7% 5.5% 4.8%% 4.8% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.007 0.082 0.164 0.264 0.521 0.984 1.692 2.055 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.007 0.082 0.164 0.264 0.521 0.984 1.692 2.055 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.007 0.082 0.164 0.264 0.521 0.984 1.692 2.055 O.D. 0.007 0.082 0.164 0.264 0.521 0.984 1.692 2.055

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.