Rat Sclerostin/SOST ELISA Kit PicoKine®

Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 37 35 34 1.47 4.3% Sample 2 214 226 230 233 225 7.22 3.2% Sample 3 472 504 525 455 489 27.23 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 37 35 34 1.47 4.3% Sample 1 33 34 37 35 34 1.47 4.3% Sample 2 214 226 230 233 225 7.22 3.2% Sample 2 214 226 230 233 225 7.22 3.2% Sample 3 472 504 525 455 489 27.23 5.5% Sample 3 472 504 525 455 489 27.23 5.5%

Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Metabolism, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Skeletal Development, Stem Cells, Structures

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 214 472 33 234 430 Standard deviation 1.78 13.26 20.29 1.94 14.97 20.64 CV (%) 5.4% 6.2% 4.3% 5.9% 6.4% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 214 472 33 234 430 Standard deviation 1.78 13.26 20.29 1.94 14.97 20.64 CV (%) 5.4% 6.2% 4.3% 5.9% 6.4% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 214 472 33 234 430 Mean (pg/ml) 33 214 472 33 234 430 Standard deviation 1.78 13.26 20.29 1.94 14.97 20.64 Standard deviation 1.78 13.26 20.29 1.94 14.97 20.64 CV (%) 5.4% 6.2% 4.3% 5.9% 6.4% 4.8% CV (%) 5.4% 6.2% 4.3% 5.9% 6.4% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.22 0.305 0.462 0.78 1.196 1.657 2.031 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.22 0.305 0.462 0.78 1.196 1.657 2.031 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.22 0.305 0.462 0.78 1.196 1.657 2.031 O.D. 0.095 0.22 0.305 0.462 0.78 1.196 1.657 2.031

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.