Rat beta IG-H3/TGFBI ELISA Kit PicoKine®

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 274 298 308 297 13.89 4.6% Sample 2 1355 1497 1581 1353 1446 97.15 6.7% Sample 3 5623 6215 5716 5905 5864 226.3 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 274 298 308 297 13.89 4.6% Sample 1 308 274 298 308 297 13.89 4.6% Sample 2 1355 1497 1581 1353 1446 97.15 6.7% Sample 2 1355 1497 1581 1353 1446 97.15 6.7% Sample 3 5623 6215 5716 5905 5864 226.3 3.8% Sample 3 5623 6215 5716 5905 5864 226.3 3.8%

Collagen, Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth Factors/Hormones, Neuroscience, Organogenesis, Sensory System, Signal Transduction, Structures, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1355 5623 336 1296 5160 Standard deviation 20.63 73.17 314.88 24.19 84.24 309.6 CV (%) 6.7% 5.4% 5.6% 7.2% 6.5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1355 5623 336 1296 5160 Standard deviation 20.63 73.17 314.88 24.19 84.24 309.6 CV (%) 6.7% 5.4% 5.6% 7.2% 6.5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 308 1355 5623 336 1296 5160 Mean (pg/ml) 308 1355 5623 336 1296 5160 Standard deviation 20.63 73.17 314.88 24.19 84.24 309.6 Standard deviation 20.63 73.17 314.88 24.19 84.24 309.6 CV (%) 6.7% 5.4% 5.6% 7.2% 6.5% 6% CV (%) 6.7% 5.4% 5.6% 7.2% 6.5% 6%

Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.037 0.092 0.22 0.512 1.046 1.577 2.032 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.037 0.092 0.22 0.512 1.046 1.577 2.032 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.037 0.092 0.22 0.512 1.046 1.577 2.032 O.D. 0.014 0.037 0.092 0.22 0.512 1.046 1.577 2.032

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.