Human FGF1/Fgf Acidic ELISA Kit PicoKine®

Human FGF1/Fgf Acidic ELISA Kit PicoKine® (96 Tests) . Quantitate Human FGF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 56 57 57 57 1.08 1.8% Sample 2 282 275 268 260 271 8.16 3% Sample 3 984 901 918 957 940 32.51 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 56 57 57 57 1.08 1.8% Sample 1 59 56 57 57 57 1.08 1.8% Sample 2 282 275 268 260 271 8.16 3% Sample 2 282 275 268 260 271 8.16 3% Sample 3 984 901 918 957 940 32.51 3.4% Sample 3 984 901 918 957 940 32.51 3.4%

Synonyms

Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1; FGF1; FGFA

Gene Name

FGF1

Gene ID

2246

UniProt

P05230

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-D155

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 282 984 58 270 1073 Standard deviation 3.3 15.42 76.75 3.82 18.9 98.71 CV (%) 5.4% 5.4% 7.8% 6.6% 7% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 282 984 58 270 1073 Standard deviation 3.3 15.42 76.75 3.82 18.9 98.71 CV (%) 5.4% 5.4% 7.8% 6.6% 7% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 282 984 58 270 1073 Mean (pg/ml) 59 282 984 58 270 1073 Standard deviation 3.3 15.42 76.75 3.82 18.9 98.71 Standard deviation 3.3 15.42 76.75 3.82 18.9 98.71 CV (%) 5.4% 5.4% 7.8% 6.6% 7% 9.2% CV (%) 5.4% 5.4% 7.8% 6.6% 7% 9.2%

Sensitivity

<10 pg/mL

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17460 MW

Specificity

Natural and recombinant human FGF1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fibroblast growth factor 1

Subcellular Location

Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu (2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity) . Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. .

Protein Name

Fibroblast growth factor 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.157 0.231 0.351 0.536 0.962 1.478 1.981 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.157 0.231 0.351 0.536 0.962 1.478 1.981 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.157 0.231 0.351 0.536 0.962 1.478 1.981 O.D. 0.032 0.157 0.231 0.351 0.536 0.962 1.478 1.981

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.