Mouse CCL27/CTACK ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK) . The human gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. And CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 178 200 189 186 8.69 4.6% Sample 2 414 438 402 375 407 22.68 5.5% Sample 3 1713 1708 1666 1742 1707 27.12 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 178 200 189 186 8.69 4.6% Sample 1 180 178 200 189 186 8.69 4.6% Sample 2 414 438 402 375 407 22.68 5.5% Sample 2 414 438 402 375 407 22.68 5.5% Sample 3 1713 1708 1666 1742 1707 27.12 1.5% Sample 3 1713 1708 1666 1742 1707 27.12 1.5%

C-C motif chemokine 27; CC chemokine ILC; Cutaneous T-cell-attracting chemokine; CTACK; ESkine; IL-11 R-alpha-locus chemokine; ALP; mILC; Skinkine; Small-inducible cytokine A27; Ccl27; Ilc, Scya27

Isoform 1 is predominantly expressed in placenta and weakly in skin. Isoform 2 is predominantly expressed in testes and brain, weakly in kidney and liver and even lower in heart and muscle. Low expression of both isoforms in other tissues.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 414 1713 188 393 1657 Standard deviation 9.18 24.01 99.35 11.84 29.47 107.7 CV (%) 6.5%% 5.8% 5.8% 7.5%% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 414 1713 188 393 1657 Standard deviation 9.18 24.01 99.35 11.84 29.47 107.7 CV (%) 6.5%% 5.8% 5.8% 7.5%% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 180 414 1713 188 393 1657 Mean (pg/ml) 180 414 1713 188 393 1657 Standard deviation 9.18 24.01 99.35 11.84 29.47 107.7 Standard deviation 9.18 24.01 99.35 11.84 29.47 107.7 CV (%) 6.5%% 5.8% 5.8% 7.5%% 7.5% 6.5% CV (%) 6.5%% 5.8% 5.8% 7.5%% 7.5% 6.5%

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.154 0.217 0.343 0.575 1.063 1.696 2.048 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.154 0.217 0.343 0.575 1.063 1.696 2.048 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.154 0.217 0.343 0.575 1.063 1.696 2.048 O.D. 0.068 0.154 0.217 0.343 0.575 1.063 1.696 2.048

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.