Rat Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®

Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH) . JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 150 175 152 164 13.38 8.1% Sample 2 515 494 479 531 504 19.82 3.9% Sample 3 1176 1181 1090 1127 1143 37.4 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 150 175 152 164 13.38 8.1% Sample 1 180 150 175 152 164 13.38 8.1% Sample 2 515 494 479 531 504 19.82 3.9% Sample 2 515 494 479 531 504 19.82 3.9% Sample 3 1176 1181 1090 1127 1143 37.4 3.2% Sample 3 1176 1181 1090 1127 1143 37.4 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 515 1176 180 499 1182 Standard deviation 10.44 29.87 56.44 11.34 34.93 74.46 CV (%) 5.8% 5.8% 4.8% 7%% 7% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 515 1176 180 499 1182 Standard deviation 10.44 29.87 56.44 11.34 34.93 74.46 CV (%) 5.8% 5.8% 4.8% 7%% 7% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 180 515 1176 180 499 1182 Mean (pg/ml) 180 515 1176 180 499 1182 Standard deviation 10.44 29.87 56.44 11.34 34.93 74.46 Standard deviation 10.44 29.87 56.44 11.34 34.93 74.46 CV (%) 5.8% 5.8% 4.8% 7%% 7% 6.3% CV (%) 5.8% 5.8% 4.8% 7%% 7% 6.3%

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.123 0.184 0.242 0.402 0.699 1.271 1.872 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.123 0.184 0.242 0.402 0.699 1.271 1.872 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.123 0.184 0.242 0.402 0.699 1.271 1.872 O.D. 0.052 0.123 0.184 0.242 0.402 0.699 1.271 1.872

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.