Mouse EPCR ELISA Kit PicoKine®

Endothelial protein C receptor (EPCR), also known as CCCA or PROCR, is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 249 247 239 243 4.32 1.7% Sample 2 1117 1152 1174 1045 1122 48.88 4.3% Sample 3 4948 5526 5362 4932 5192 258.64 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 249 247 239 243 4.32 1.7% Sample 1 240 249 247 239 243 4.32 1.7% Sample 2 1117 1152 1174 1045 1122 48.88 4.3% Sample 2 1117 1152 1174 1045 1122 48.88 4.3% Sample 3 4948 5526 5362 4932 5192 258.64 4.9% Sample 3 4948 5526 5362 4932 5192 258.64 4.9%

Endothelial protein C receptor; Activated protein C receptor; APC receptor; Centrocyclin; Centrosomal protein CCD41; Endothelial cell protein C receptor; CD201; Procr; Epcr

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1117 4948 231 1074 4505 Standard deviation 14.4 54.73 366.15 16.4 55.84 351.39 CV (%) 6% 4.9% 7.4% 7.1% 5.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1117 4948 231 1074 4505 Standard deviation 14.4 54.73 366.15 16.4 55.84 351.39 CV (%) 6% 4.9% 7.4% 7.1% 5.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 240 1117 4948 231 1074 4505 Mean (pg/ml) 240 1117 4948 231 1074 4505 Standard deviation 14.4 54.73 366.15 16.4 55.84 351.39 Standard deviation 14.4 54.73 366.15 16.4 55.84 351.39 CV (%) 6% 4.9% 7.4% 7.1% 5.2% 7.8% CV (%) 6% 4.9% 7.4% 7.1% 5.2% 7.8%

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.071 0.121 0.228 0.45 0.935 1.793 2.57 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.071 0.121 0.228 0.45 0.935 1.793 2.57 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.071 0.121 0.228 0.45 0.935 1.793 2.57 O.D. 0.019 0.071 0.121 0.228 0.45 0.935 1.793 2.57

Activate the sample Cell culture supernatants, serum and plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 25μl of Solution A into 50μl of sample, 10 min later, add 25μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant EPCR. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants, serum and plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 25μl of Solution A into 50μl of sample, 10 min later, add 25μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant EPCR. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.