Mouse Meteorin/METRN ELISA Kit PicoKine®

Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts ly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 115 103 111 109 4.38 4% Sample 2 653 598 604 672 631 31.54 4.9% Sample 3 1218 1142 1126 1142 1157 35.81 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 115 103 111 109 4.38 4% Sample 1 108 115 103 111 109 4.38 4% Sample 2 653 598 604 672 631 31.54 4.9% Sample 2 653 598 604 672 631 31.54 4.9% Sample 3 1218 1142 1126 1142 1157 35.81 3% Sample 3 1218 1142 1126 1142 1157 35.81 3%

Highly expressed in brain. Expressed in undifferentiated neural progenitors and in astrocyte lineage, particulary in Bergmann glia, a subtype of radial glia, and a few discrete neuronal populations residing in the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and in various thalamic nuclei. Weakly expressed in heart, kidney, skeletal muscle, spleen, testis, gut and lung. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 653 1218 110 595 1211 Standard deviation 5.83 50.28 58.46 6.05 52.95 76.29 CV (%) 5.4% 7.7% 4.8% 5.5% 8.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 653 1218 110 595 1211 Standard deviation 5.83 50.28 58.46 6.05 52.95 76.29 CV (%) 5.4% 7.7% 4.8% 5.5% 8.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 653 1218 110 595 1211 Mean (pg/ml) 108 653 1218 110 595 1211 Standard deviation 5.83 50.28 58.46 6.05 52.95 76.29 Standard deviation 5.83 50.28 58.46 6.05 52.95 76.29 CV (%) 5.4% 7.7% 4.8% 5.5% 8.9% 6.3% CV (%) 5.4% 7.7% 4.8% 5.5% 8.9% 6.3%

Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.