Mouse HAI-2/SPINT2 ELISA Kit PicoKine®

Mouse HAI-2/SPINT2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Spint2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 62 57 60 1.92 3.2% Sample 2 240 243 264 249 249 9.24 3.7% Sample 3 837 921 842 963 890 53.38 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 62 57 60 1.92 3.2% Sample 1 61 61 62 57 60 1.92 3.2% Sample 2 240 243 264 249 249 9.24 3.7% Sample 2 240 243 264 249 249 9.24 3.7% Sample 3 837 921 842 963 890 53.38 5.9% Sample 3 837 921 842 963 890 53.38 5.9%

Synonyms

Kunitz-type protease inhibitor 2; Hepatocyte growth factor activator inhibitor type 2; HAI-2; Spint2; Hai2

Gene Name

Spint2

Gene ID

20733

UniProt

Q9WU03

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R30-K140

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Isoform 2 is more predominantly expressed than isoform 1.

Applications

ELISA

Field of Research

Cell Biology, Developmental Biology, Neurogenesis, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Reproduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 240 837 64 248 772 Mean (pg/ml) 61 240 837 64 248 772 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 Standard deviation 4.14 13.92 46.03 5.56 17.85 55.58 CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2% CV (%) 6.8% 5.8% 5.5% 8.7% 7.2% 7.2%

Sensitivity

<10 pg/mL

Function

Inhibitor of HGF activator.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27914 MW

Specificity

Natural and recombinant mouse HAI-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kunitz-type protease inhibitor 2

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Kunitz-type protease inhibitor 2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395 O.D. 0.036 0.172 0.245 0.391 0.618 1.017 1.57 2.395

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.