Mouse Kremen-1 ELISA Kit PicoKine®

Mouse Kremen-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Kremen1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kremen protein 1 is a protein that in humans is encoded by the KREMEN1 gene. This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT) /beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6) . It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 171 172 159 168 5.35 3.1% Sample 2 880 925 969 991 941 42.6 4.5% Sample 3 4010 4034 3878 4402 4081 194.61 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 171 172 159 168 5.35 3.1% Sample 1 171 171 172 159 168 5.35 3.1% Sample 2 880 925 969 991 941 42.6 4.5% Sample 2 880 925 969 991 941 42.6 4.5% Sample 3 4010 4034 3878 4402 4081 194.61 4.7% Sample 3 4010 4034 3878 4402 4081 194.61 4.7%

Synonyms

Kremen protein 1; Dickkopf receptor; Kringle domain-containing transmembrane protein 1; Kringle-containing protein marking the eye and the nose; Kremen1; Kremen

Gene Name

Kremen1

Gene ID

84035

UniProt

Q99N43

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-G395

Tissue Specificity

In the adult, widely expressed with high levels in heart, lung, kidney, skeletal muscle and testis. .

Applications

ELISA

Field of Research

Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Signaling Pathways, Stem Cells, Surface Molecules

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 171 880 4010 187 831 3851 Mean (pg/ml) 171 880 4010 187 831 3851 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 Standard deviation 6.84 57.2 308.77 8.41 59 323.48 CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4% CV (%) 4% 6.5% 7.7% 4.5% 7.1% 8.4%

Sensitivity

<10 pg/mL

Function

Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

51673 MW

Specificity

Natural and recombinant mouse Kremen-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kremen protein 1

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Kremen protein 1

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125 O.D. 0.042 0.117 0.204 0.345 0.641 1.14 1.612 2.125

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.