Human Nectin-4/PVRL4 ELISA Kit PicoKine®

Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Sample 3 857 1006 836 933 908 67.1 7.3%

Nectin-4; Ig superfamily receptor LNIR; Nectin cell adhesion molecule 4 ; Poliovirus receptor-related protein 4; Processed poliovirus receptor-related protein 4; NECTIN4 ; LNIR, PRR4, PVRL4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3% CV (%) 4.9% 7.2%% 5.2% 5.9% 7.2% 5.3%

Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with MLLT4 at cadherin-based adherens junctions (PubMed:11544254) .

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902 O.D. 0.028 0.086 0.133 0.207 0.375 0.6251 1.118 1.902

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.